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Addgene

Scarlet I-FRET 10-NeonGreen
(Plasmid #139364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scarlet 10AA Neon green
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Mfe1 (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer CTC TAC AAA TGT GGT ATG G
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

we used #98839 (mScarlet-I-mTurquoise2) as a base for the vector and replaced Turquoise2 with Neon green.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Scarlet I-FRET 10-NeonGreen was a gift from Richard Day (Addgene plasmid # 139364 ; http://n2t.net/addgene:139364 ; RRID:Addgene_139364)

  • For your References section:

    PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells. Reissaus CA, Day KH, Mirmira RG, Dunn KW, Pavalko FM, Day RN. Biophys J. 2020 Apr 21;118(8):1820-1829. doi: 10.1016/j.bpj.2020.03.003. Epub 2020 Mar 10. 10.1016/j.bpj.2020.03.003 PubMed 32191861
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