Skip to main content
Addgene

pRC/CMV-JAK1K908E-VSV
(Plasmid #139357)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRC/CMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JAK1 cDNA with 300nt of 5' UTR
  • Species
    H. sapiens (human)
  • Mutation
    K908E
  • Entrez Gene
    JAK1 (a.k.a. AIIDE, JAK1A, JAK1B, JTK3)
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AfeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original JAK1 cDNA clone from Andrew Wilks, Ludwig Institute for Cancer Research, Victoria, Australia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC/CMV-JAK1K908E-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139357 ; http://n2t.net/addgene:139357 ; RRID:Addgene_139357)

  • For your References section:

    Two rare disease-associated Tyk2 variants are catalytically impaired but signaling competent. Li Z, Gakovic M, Ragimbeau J, Eloranta ML, Ronnblom L, Michel F, Pellegrini S. J Immunol. 2013 Mar 1;190(5):2335-44. doi: 10.4049/jimmunol.1203118. Epub 2013 Jan 28. 10.4049/jimmunol.1203118 PubMed 23359498
Related items:
Commonly requested with: