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Addgene

pRC/CMV-JAK1-VSV
(Plasmid #139356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRC/CMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JAK1 cDNA with 300nt of 5'UTR
  • Species
    H. sapiens (human)
  • Entrez Gene
    JAK1 (a.k.a. AIIDE, JAK1A, JAK1B, JTK3)
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original JAK1 cDNA clone from Andrew Wilks, Ludwig Institute for Cancer Research, Victoria, Australia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC/CMV-JAK1-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139356 ; http://n2t.net/addgene:139356 ; RRID:Addgene_139356)

  • For your References section:

    Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. Richter MF, Dumenil G, Uze G, Fellous M, Pellegrini S. J Biol Chem. 1998 Sep 18;273(38):24723-9. doi: 10.1074/jbc.273.38.24723. 10.1074/jbc.273.38.24723 PubMed 9733772
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