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pEGFP-N3-Myr-TYK2
(Plasmid #139355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus
  • Species
    H. sapiens (human)
  • Entrez Gene
    TYK2 (a.k.a. IMD35, JTK1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • aa 1-16 of human Lck (N terminal on insert)
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N3-Myr-TYK2 was a gift from Sandra Pellegrini (Addgene plasmid # 139355 ; http://n2t.net/addgene:139355 ; RRID:Addgene_139355)

  • For your References section:

    The receptor interaction region of Tyk2 contains a motif required for its nuclear localization. Ragimbeau J, Dondi E, Vasserot A, Romero P, Uze G, Pellegrini S. J Biol Chem. 2001 Aug 17;276(33):30812-8. doi: 10.1074/jbc.M103559200. Epub 2001 Jun 8. 10.1074/jbc.M103559200 PubMed 11399767
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