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pRC/CMV-TYK2V678F-VSV
(Plasmid #139349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRC/CMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TYK2 cDNA with 267nt of 5' UTR
  • Species
    H. sapiens (human)
  • Mutation
    V362F-V678F
  • Entrez Gene
    TYK2 (a.k.a. IMD35, JTK1)
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site BstXI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC/CMV-TYK2V678F-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139349 ; http://n2t.net/addgene:139349 ; RRID:Addgene_139349)

  • For your References section:

    The Stat3-activating Tyk2 V678F mutant does not up-regulate signaling through the type I interferon receptor but confers ligand hypersensitivity to a homodimeric receptor. Gakovic M, Ragimbeau J, Francois V, Constantinescu SN, Pellegrini S. J Biol Chem. 2008 Jul 4;283(27):18522-9. doi: 10.1074/jbc.M801427200. Epub 2008 May 2. 10.1074/jbc.M801427200 PubMed 18456658
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