pRC/CMV-TYK2K930R-FF-VSV
(Plasmid
#139348)
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PurposeExpression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRC/CMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTYK2 cDNA with 267nt of 5' UTR
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SpeciesH. sapiens (human)
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MutationV362F-K930R-Y1054F-Y1055F
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Entrez GeneTYK2 (a.k.a. IMD35, JTK1)
- Promoter CMV
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Tag
/ Fusion Protein
- VSV (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC/CMV-TYK2K930R-FF-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139348 ; http://n2t.net/addgene:139348 ; RRID:Addgene_139348) -
For your References section:
Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase. Gauzzi MC, Velazquez L, McKendry R, Mogensen KE, Fellous M, Pellegrini S. J Biol Chem. 1996 Aug 23;271(34):20494-500. doi: 10.1074/jbc.271.34.20494. 10.1074/jbc.271.34.20494 PubMed 8702790
