Skip to main content
Addgene

pRC/CMV-TYK2-VSV
(Plasmid #139344)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRC/CMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TYK2 cDNA with 267nt of 5' UTR
  • Species
    H. sapiens (human)
  • Entrez Gene
    TYK2 (a.k.a. IMD35, JTK1)
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC/CMV-TYK2-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139344 ; http://n2t.net/addgene:139344 ; RRID:Addgene_139344)

  • For your References section:

    Two common disease-associated TYK2 variants impact exon splicing and TYK2 dosage. Li Z, Rotival M, Patin E, Michel F, Pellegrini S. PLoS One. 2020 Jan 21;15(1):e0225289. doi: 10.1371/journal.pone.0225289. eCollection 2020. 10.1371/journal.pone.0225289 PubMed 31961910
Related items:
Commonly requested with: