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Addgene

pUC57-Curlcake2
(Plasmid #139341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Curlcake2 with T7 promoter
  • Alt name
    CC2
  • Species
    Synthetic
  • Insert Size (bp)
    2459
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-Curlcake2 was a gift from Eva Maria Novoa (Addgene plasmid # 139341 ; http://n2t.net/addgene:139341 ; RRID:Addgene_139341)

  • For your References section:

    Accurate detection of m(6)A RNA modifications in native RNA sequences. Liu H, Begik O, Lucas MC, Ramirez JM, Mason CE, Wiener D, Schwartz S, Mattick JS, Smith MA, Novoa EM. Nat Commun. 2019 Sep 9;10(1):4079. doi: 10.1038/s41467-019-11713-9. 10.1038/s41467-019-11713-9 PubMed 31501426
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