Akap1(31)-FRB-iRFP
(Plasmid
#139315)
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Purposemitochondrial-resident FRB for chemically induced dimerization
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepiRFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
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SpeciesM. musculus (mouse)
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MutationM16L
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The M16L mutation in the plasmid insert eliminates an alternative start codon that leads to an ER-targeted protein.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Akap1(31)-FRB-iRFP was a gift from Gerry Hammond (Addgene plasmid # 139315 ; http://n2t.net/addgene:139315 ; RRID:Addgene_139315) -
For your References section:
Probing the subcellular distribution of phosphatidylinositol reveals a surprising lack at the plasma membrane. Zewe JP, Miller AM, Sangappa S, Wills RC, Goulden BD, Hammond GRV. J Cell Biol. 2020 Mar 2;219(3). pii: 133808. doi: 10.1083/jcb.201906127. 10.1083/jcb.201906127 PubMed 32211893
