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Addgene

Akap1(31)-FRB-iRFP
(Plasmid #139315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piRFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
  • Species
    M. musculus (mouse)
  • Mutation
    M16L

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The M16L mutation in the plasmid insert eliminates an alternative start codon that leads to an ER-targeted protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Akap1(31)-FRB-iRFP was a gift from Gerry Hammond (Addgene plasmid # 139315 ; http://n2t.net/addgene:139315 ; RRID:Addgene_139315)

  • For your References section:

    Probing the subcellular distribution of phosphatidylinositol reveals a surprising lack at the plasma membrane. Zewe JP, Miller AM, Sangappa S, Wills RC, Goulden BD, Hammond GRV. J Cell Biol. 2020 Mar 2;219(3). pii: 133808. doi: 10.1083/jcb.201906127. 10.1083/jcb.201906127 PubMed 32211893
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