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Addgene

pmCherry-C1ab-Prkd1
(Plasmid #139314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry:Mus musculus Prkd1(138-343)
  • Species
    M. musculus (mouse)
  • Mutation
    T208A, N313D
  • Entrez Gene
    Prkd1 (a.k.a. PKD, PKD1, Pkcm, Prkcm, nPKC-D1, nPKC-mu)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-C1ab-Prkd1 was a gift from Gerry Hammond (Addgene plasmid # 139314 ; http://n2t.net/addgene:139314 ; RRID:Addgene_139314)

  • For your References section:

    Probing the subcellular distribution of phosphatidylinositol reveals a surprising lack at the plasma membrane. Zewe JP, Miller AM, Sangappa S, Wills RC, Goulden BD, Hammond GRV. J Cell Biol. 2020 Mar 2;219(3). pii: 133808. doi: 10.1083/jcb.201906127. 10.1083/jcb.201906127 PubMed 32211893
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