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Addgene

pBMN-AS-HNF4α
(Plasmid #139305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139305 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN(CMV-copGFP-Puro)
  • Backbone manufacturer
    Constructed from pGreenPuro (SBI) by Magnus Essand Lab
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    shRNA and amiRNA against HNF4α
  • gRNA/shRNA sequence
    shRNA targeting "GAACCACATGTACTCCTGCAGATT" and amiRNA targeting "TCAGCACTCGAAGGTCAAGCTAT" of human HNF4α mRNA
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN-AS-HNF4α was a gift from Claes Wadelius (Addgene plasmid # 139305 ; http://n2t.net/addgene:139305 ; RRID:Addgene_139305)

  • For your References section:

    rs953413 Regulates Polyunsaturated Fatty Acid Metabolism by Modulating ELOVL2 Expression. Pan G, Cavalli M, Carlsson B, Skrtic S, Kumar C, Wadelius C. iScience. 2020 Jan 9:100808. doi: 10.1016/j.isci.2019.100808. 10.1016/j.isci.2019.100808 PubMed 31928966
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