pBMN-AS-FOXA1
(Plasmid
#139304)
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PurposeKnocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBMN(CMV-copGFP-Puro)
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Backbone manufacturerConstructed from pGreenPuro (SBI) by Magnus Essand Lab
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameshRNA and amiRNA against FOXA1
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gRNA/shRNA sequenceshRNA targeting "GATGTGTAGACATCCTCCGTATATT" and amiRNA targeting "GGCGTACTACCAAGGTGTGTA" of human FOXA1 mRNA
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN-AS-FOXA1 was a gift from Claes Wadelius (Addgene plasmid # 139304 ; http://n2t.net/addgene:139304 ; RRID:Addgene_139304) -
For your References section:
rs953413 Regulates Polyunsaturated Fatty Acid Metabolism by Modulating ELOVL2 Expression. Pan G, Cavalli M, Carlsson B, Skrtic S, Kumar C, Wadelius C. iScience. 2020 Jan 9:100808. doi: 10.1016/j.isci.2019.100808. 10.1016/j.isci.2019.100808 PubMed 31928966