cfmTurquoise-N
(Plasmid
#139284)
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PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139284 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemTurquoise-N1
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Backbone manufacturerClontech
- Total vector size (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecfmTurquoise
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SpeciesSynthetic
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Insert Size (bp)720
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MutationCys48Ser and Cys70/Met
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Tag
/ Fusion Protein
- Multi-cloning sites (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cfmTurquoise-N was a gift from Ikuo Wada (Addgene plasmid # 139284 ; http://n2t.net/addgene:139284 ; RRID:Addgene_139284) -
For your References section:
Integrative genome analysis identified the KANNO blood group antigen as prion protein. Omae Y, Ito S, Takeuchi M, Isa K, Ogasawara K, Kawabata K, Oda A, Kaito S, Tsuneyama H, Uchikawa M, Wada I, Ohto H, Tokunaga K. Transfusion. 2019 Jul;59(7):2429-2435. doi: 10.1111/trf.15319. Epub 2019 Apr 24. 10.1111/trf.15319 PubMed 31020675
