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pET28a-SznE
(Plasmid #139282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5308
  • Total vector size (bp) 6394
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sznE
  • Species
    Streptomyces achromogenes subsp. streptozoticus
  • Insert Size (bp)
    1086
  • GenBank ID
    MK291259.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SznE was a gift from Emily Balskus (Addgene plasmid # 139282 ; http://n2t.net/addgene:139282 ; RRID:Addgene_139282)

  • For your References section:

    An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin. Ng TL, Rohac R, Mitchell AJ, Boal AK, Balskus EP. Nature. 2019 Feb;566(7742):94-99. doi: 10.1038/s41586-019-0894-z. Epub 2019 Feb 6. 10.1038/s41586-019-0894-z PubMed 30728519
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