pB6-Id1-T2A-tdTomato-FNF-TK
(Plasmid #139281)

• Purpose: B6J Id1-T2A-tdTomato allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: pWS-TK6
• Backbone manufacturer: Sen Wu, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Id1
• Species: M. musculus (mouse)
• Entrez Gene: Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
• Tag / Fusion Protein:
  • C-terminal fusion of T2A-tdTomato (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pId1-T2A-tdTomato-TK.gb
• A portion of this plasmid was derived from a plasmid made by: tdTomato is from Roger Tsien. FRT-Neo-FRT is from pFNF (#22687).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive A-G region. Depositor confirms these bases do not affect plasmid function.