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Addgene

pB6-Id1-T2A-tdTomato-FNF-TK
(Plasmid #139281)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139281 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWS-TK6
  • Backbone manufacturer
    Sen Wu, Capecchi laboratory
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Id1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Tag / Fusion Protein
    • C-terminal fusion of T2A-tdTomato (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    tdTomato is from Roger Tsien. FRT-Neo-FRT is from pFNF (#22687).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive A-G region. Depositor confirms these bases do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-Id1-T2A-tdTomato-FNF-TK was a gift from Mario Capecchi (Addgene plasmid # 139281 ; http://n2t.net/addgene:139281 ; RRID:Addgene_139281)