PB ID1-iCreERT2
(Plasmid #139280)

• Purpose: Human ID1-iCreERT2 transgene piggyBac transposon
• Depositing Lab: Mario Capecchi
• Publication: piggyBac transposon plasmids (unpublished)

Backbone

• Vector backbone: p15A-PB
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ID1
• Species: H. sapiens (human)
• Mutation: Replaced the ID1 protein coding sequence in the two exons with the iCreERT2 cDNA intervened by the endogenous ID1 intron
• Entrez Gene: ID1 (a.k.a. ID, bHLHb24)

Resource Information

• Supplemental Documents:
  • p15A-PB-ID1-iCreERT2.gb
  • Human ID1 iCreERT2 PB transgene
• A portion of this plasmid was derived from a plasmid made by: iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.

The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.