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Addgene

PB ID1-iCreERT2
(Plasmid #139280)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139280 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p15A-PB
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ID1
  • Species
    H. sapiens (human)
  • Mutation
    Replaced the ID1 protein coding sequence in the two exons with the iCreERT2 cDNA intervened by the endogenous ID1 intron
  • Entrez Gene
    ID1 (a.k.a. ID, bHLHb24)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.

The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB ID1-iCreERT2 was a gift from Mario Capecchi (Addgene plasmid # 139280 ; http://n2t.net/addgene:139280 ; RRID:Addgene_139280)