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Addgene

RCAS (A) Flu cPitx2 (CT#704)
(Plasmid #13928)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pitx2
  • Alt name
    Ptx2
  • Species
    G. gallus (chicken)
  • Entrez Gene
    PITX2
  • Tag / Fusion Protein
    • Flu (3xHA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) Flu cPitx2 (CT#704) was a gift from Cliff Tabin (Addgene plasmid # 13928 ; http://n2t.net/addgene:13928 ; RRID:Addgene_13928)

  • For your References section:

    The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals. Logan M, Pagan-Westphal SM, Smith DM, Paganessi L, Tabin CJ. Cell. 1998 Aug 7. 94(3):307-17. 10.1016/S0092-8674(00)81474-9 PubMed 9708733
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