RCAS (A) Flu cPitx2 (CT#704)
(Plasmid
#13928)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepitx2
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Alt namePtx2
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SpeciesG. gallus (chicken)
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Entrez GenePITX2
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Tag
/ Fusion Protein
- Flu (3xHA) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (A) Flu cPitx2 (CT#704) was a gift from Cliff Tabin (Addgene plasmid # 13928 ; http://n2t.net/addgene:13928 ; RRID:Addgene_13928) -
For your References section:
The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals. Logan M, Pagan-Westphal SM, Smith DM, Paganessi L, Tabin CJ. Cell. 1998 Aug 7. 94(3):307-17. 10.1016/S0092-8674(00)81474-9 PubMed 9708733
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/43/60/9ca6d80a-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)