pBS cPitx2 (CT#73)
(Plasmid
#13927)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepitx2
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SpeciesG. gallus (chicken)
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Insert Size (bp)1700
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Entrez GenePITX2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
full length original library isolate from Mackem dT, stage18-24 limb bud library. Used as an anti-sense riboprobe template and for subcloning.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cPitx2 (CT#73) was a gift from Cliff Tabin (Addgene plasmid # 13927 ; http://n2t.net/addgene:13927 ; RRID:Addgene_13927) -
For your References section:
The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals. Logan M, Pagan-Westphal SM, Smith DM, Paganessi L, Tabin CJ. Cell. 1998 Aug 7. 94(3):307-17. 10.1016/S0092-8674(00)81474-9 PubMed 9708733