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Addgene

RCAS (A) Flu cTbx4 (CT#700)
(Plasmid #13925)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tbx4
  • Species
    G. gallus (chicken)
  • GenBank ID
    AF069395
  • Entrez Gene
    TBX4
  • Tag / Fusion Protein
    • Flu (3xHA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Flu-tagged (c-terminal) Tbx4 retroviral construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) Flu cTbx4 (CT#700) was a gift from Cliff Tabin (Addgene plasmid # 13925 ; http://n2t.net/addgene:13925 ; RRID:Addgene_13925)

  • For your References section:

    Differential regulation of T-box and homeobox transcription factors suggests roles in controlling chick limb-type identity. Logan M, Simon HG, Tabin C. Development. 1998 Aug . 125(15):2825-35. PubMed 9655805
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