pBS cTbx4 (CT#137)
(Plasmid
#13924)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTbx4
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SpeciesG. gallus (chicken)
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GenBank IDAF069395
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Entrez GeneTBX4
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length Tbx4 ORF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cTbx4 (CT#137) was a gift from Cliff Tabin (Addgene plasmid # 13924 ; http://n2t.net/addgene:13924 ; RRID:Addgene_13924) -
For your References section:
Differential regulation of T-box and homeobox transcription factors suggests roles in controlling chick limb-type identity. Logan M, Simon HG, Tabin C. Development. 1998 Aug . 125(15):2825-35. PubMed 9655805