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Addgene

RCAS (A) cTbx3 del2 (CT#645)
(Plasmid #13921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tbx3 del
  • Alt name
    Tbx3
  • Species
    G. gallus (chicken)
  • Mutation
    Truncated Tbx3. Putative dominant negative.
  • GenBank ID
    AF069394
  • Entrez Gene
    TBX3 (a.k.a. TBX3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) cTbx3 del2 (CT#645) was a gift from Cliff Tabin (Addgene plasmid # 13921 ; http://n2t.net/addgene:13921 ; RRID:Addgene_13921)

  • For your References section:

    Differential regulation of T-box and homeobox transcription factors suggests roles in controlling chick limb-type identity. Logan M, Simon HG, Tabin C. Development. 1998 Aug . 125(15):2825-35. PubMed 9655805
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