pHA851 - osm-10p::Fynempty
(Plasmid
#139208)
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PurposeControl vector for Fyn kinase expression in osm-10 neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFyn
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SpeciesH. sapiens (human)
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Mutationdeletion and frame shift to prevent Fyn expression
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Entrez GeneFYN (a.k.a. SLK, SYN, p59-FYN)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHA851 - osm-10p::Fynempty was a gift from Anne Hart (Addgene plasmid # 139208 ; http://n2t.net/addgene:139208 ; RRID:Addgene_139208) -
For your References section:
Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration. Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, Mittal J, Hart AC, Fawzi NL. EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. Epub 2020 Dec 22. 10.15252/embj.2020105001 PubMed 33349959