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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet
(Plasmid #139205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hrp-1
  • Species
    C. elegans (nematode)
  • Mutation
    mScarlet added between RRMs and LC, last exon replaced with corresponding human sequence
  • Entrez Gene
    hrp-1 (a.k.a. CELE_F42A6.7)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHA845 - hrp-1p::hrp-1HsLCWTmScarlet was a gift from Anne Hart (Addgene plasmid # 139205 ; http://n2t.net/addgene:139205 ; RRID:Addgene_139205)

  • For your References section:

    Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration. Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, Mittal J, Hart AC, Fawzi NL. EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. Epub 2020 Dec 22. 10.15252/embj.2020105001 PubMed 33349959
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