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Addgene

pcDNA3.1 Mfn2-HA S27A
(Plasmid #139193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7766
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MFN2
  • Alt name
    Mitofusin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2273
  • Mutation
    Serine 27 to Alanine
  • GenBank ID
    NM_014874
  • Entrez Gene
    MFN2 (a.k.a. CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF, MSL)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • 3HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer pCDNA3.1/BGH
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Mfn2-HA S27A was a gift from Allan Weissman (Addgene plasmid # 139193 ; http://n2t.net/addgene:139193 ; RRID:Addgene_139193)

  • For your References section:

    Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis. Leboucher GP, Tsai YC, Yang M, Shaw KC, Zhou M, Veenstra TD, Glickman MH, Weissman AM. Mol Cell. 2012 Aug 24;47(4):547-57. doi: 10.1016/j.molcel.2012.05.041. Epub 2012 Jun 28. 10.1016/j.molcel.2012.05.041 PubMed 22748923
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