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Addgene

pSB-Bleo Gal4 tBFP
(Plasmid #139190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 5230
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tBFP
  • Alt name
    tag BFP
  • Insert Size (bp)
    699
  • Promoter Gal4 CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
  • 3′ sequencing primer gatgagtttggacaaaccac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with Gal4 inducible tagBFP and constitutive bleomycin resistance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB-Bleo Gal4 tBFP was a gift from Neal Devaraj (Addgene plasmid # 139190 ; http://n2t.net/addgene:139190 ; RRID:Addgene_139190)
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