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Addgene

pSBBi-Pur GFP-TM
(Plasmid #139185)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSBBi-Pur
  • Backbone size w/o insert (bp) 5857
  • Total vector size (bp) 6618
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-TM
  • Alt name
    surface GFP
  • Alt name
    EGFP Ligand
  • Species
    Aequorea victoria
  • Insert Size (bp)
    933
  • Mutation
    fused to membrane anchor
  • GenBank ID
  • Promoter EF1α

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains surface-anchored EGFP; the other side contains puromycin resistance .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBBi-Pur GFP-TM was a gift from Neal Devaraj (Addgene plasmid # 139185 ; http://n2t.net/addgene:139185 ; RRID:Addgene_139185)
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