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Addgene

pSBBi-GB TCS2
(Plasmid #139171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSBBi-GB
  • Total vector size (bp) 7350
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    probable caffeine synthase 2 [Camellia sinensis]
  • Alt name
    TCS2
  • Alt name
    Tea caffeine synthase 2
  • Alt name
    7-methylxanthosine synthase
  • Species
    Coffee arabica
  • Insert Size (bp)
    1095
  • GenBank ID
    Q68CM3.1
  • Promoter EF1α

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis probable caffeine synthase 2 (TCS2); the other side contains GFP and blasticidin resistance genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBBi-GB TCS2 was a gift from Neal Devaraj (Addgene plasmid # 139171 ; http://n2t.net/addgene:139171 ; RRID:Addgene_139171)
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