pSBBi-RP GST-TCS1
(Plasmid
#139166)
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PurposeSB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSBBi-RP
- Total vector size (bp) 7521
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Vector typeMammalian Expression ; Transposon
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlutathione transferase + caffeine synthase 1 [Camellia sinensis]
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Alt nameGST-TCS1
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Alt nameGST-Tea Caffeine Synthase 1
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Alt nameTCS1
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SpeciesCamellia sinensis
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Insert Size (bp)1818
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GenBank IDBAB12278.1
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Tag
/ Fusion Protein
- glutathione transferase (GST) (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBBi-RP GST-TCS1 was a gift from Neal Devaraj (Addgene plasmid # 139166 ; http://n2t.net/addgene:139166 ; RRID:Addgene_139166)