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Addgene

pSBBi-RP GST-TCS1
(Plasmid #139166)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139166 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSBBi-RP
  • Total vector size (bp) 7521
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glutathione transferase + caffeine synthase 1 [Camellia sinensis]
  • Alt name
    GST-TCS1
  • Alt name
    GST-Tea Caffeine Synthase 1
  • Alt name
    TCS1
  • Species
    Camellia sinensis
  • Insert Size (bp)
    1818
  • GenBank ID
    BAB12278.1
  • Tag / Fusion Protein
    • glutathione transferase (GST) (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBBi-RP GST-TCS1 was a gift from Neal Devaraj (Addgene plasmid # 139166 ; http://n2t.net/addgene:139166 ; RRID:Addgene_139166)