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Addgene

pSBBi-RP GST-CCS1
(Plasmid #139164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSBBi-RP
  • Total vector size (bp) 8301
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glutathione transferase + caffeine synthase 1 [Coffea arabica]
  • Alt name
    CCS1
  • Alt name
    GST-CCS1
  • Species
    Coffee arabica
  • Insert Size (bp)
    1860
  • GenBank ID
    Q8H0D3.1
  • Promoter EF1α
  • Tag / Fusion Protein
    • Glutathione transferase (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica caffeine synthase 1 with N-terminal glutathione transferase; the other side contains RFP and puromycin resistance genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBBi-RP GST-CCS1 was a gift from Neal Devaraj (Addgene plasmid # 139164 ; http://n2t.net/addgene:139164 ; RRID:Addgene_139164)
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