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Addgene

pSBBi-RP GST-PcCS
(Plasmid #139160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSBBi-RP
  • Total vector size (bp) 8232
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-GST caffeine Synthase [Paullinia cupana var. sorbilis]
  • Alt name
    GST-PcCS
  • Alt name
    Paullinia cupana var. sorbilis Caffeine Synthase
  • Species
    Paullinia cupana
  • Insert Size (bp)
    1797
  • GenBank ID
    BK008796.1 DAA64605.1
  • Promoter EF1α
  • Tag / Fusion Protein
    • glutathione transferase (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana var. sorbilis caffeine synthase; the other side contains RFP and puromycin resistance genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBBi-RP GST-PcCS was a gift from Neal Devaraj (Addgene plasmid # 139160 ; http://n2t.net/addgene:139160 ; RRID:Addgene_139160)
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