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FUS_LC_charge_RtoK
(Plasmid #139127)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139127 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ411
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS
  • Species
    H. sapiens (human)
  • Mutation
    Add K and D to FUS LC so it has the same charge patterning as hnRNPA2 LC
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUS_LC_charge_RtoK was a gift from Nicolas Fawzi (Addgene plasmid # 139127 ; http://n2t.net/addgene:139127 ; RRID:Addgene_139127)

  • For your References section:

    Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration. Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, Mittal J, Hart AC, Fawzi NL. EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. Epub 2020 Dec 22. 10.15252/embj.2020105001 PubMed 33349959
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