pTHMT_hnRNPF_deltaPLD
(Plasmid
#139115)
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Purposeexpress FL hnRNPF without the prion like domain with N-terminal maltose binding protein tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139115 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTHMT
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehnRNPF
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SpeciesH. sapiens (human)
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Mutationdelta365-415
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Entrez GeneHNRNPF (a.k.a. HNRPF, OK/SW-cl.23, mcs94-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTHMT_hnRNPF_deltaPLD was a gift from Nicolas Fawzi (Addgene plasmid # 139115 ; http://n2t.net/addgene:139115 ; RRID:Addgene_139115) -
For your References section:
Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration. Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, Mittal J, Hart AC, Fawzi NL. EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. Epub 2020 Dec 22. 10.15252/embj.2020105001 PubMed 33349959