H446 EF1a RA-ABE6.3 p2A Puro
(Plasmid
#139104)
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PurposeLentiviral expression of ABE6.3.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelenti
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameABE RA6.3
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a
- 3′ sequencing primer Puro-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H446 EF1a RA-ABE6.3 p2A Puro was a gift from Wen Xue (Addgene plasmid # 139104 ; http://n2t.net/addgene:139104 ; RRID:Addgene_139104) -
For your References section:
Adenine base editing in an adult mouse model of tyrosinaemia. Song CQ, Jiang T, Richter M, Rhym LH, Koblan LW, Zafra MP, Schatoff EM, Doman JL, Cao Y, Dow LE, Zhu LJ, Anderson DG, Liu DR, Yin H, Xue W. Nat Biomed Eng. 2019 Feb 25. pii: 10.1038/s41551-019-0357-8. doi: 10.1038/s41551-019-0357-8. 10.1038/s41551-019-0357-8 PubMed 31740768
Map uploaded by the depositor.
