RCAS(A) mPea3 EnR (CT#1120)
(Plasmid
#13909)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 13909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
-
Vector typeRetroviral ; avian expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePea3
-
Alt nameEnR
-
SpeciesM. musculus (mouse)
-
Entrez GeneEtv4 (a.k.a. AW414408, Pea-3, Pea3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Pea3 ETS domain fused to EnR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RCAS(A) mPea3 EnR (CT#1120) was a gift from Cliff Tabin (Addgene plasmid # 13909 ; http://n2t.net/addgene:13909 ; RRID:Addgene_13909) -
For your References section:
FGF acts directly on the somitic tendon progenitors through the Ets transcription factors Pea3 and Erm to regulate scleraxis expression. Brent AE, Tabin CJ. Development. 2004 Aug . 131(16):3885-96. 10.1242/dev.01275 PubMed 15253939