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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE Citations (5)

Originally described in: High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Legut M, Daniloski Z, Xue X, McKenzie D, Guo X, Wessels HH, Sanjana NE Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
PubMed Journal

Articles Citing pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Articles
A polyclonal allelic expression assay for detecting regulatory effects of transcript variants. Brandt M, Gokden A, Ziosi M, Lappalainen T. Genome Med. 2020 Sep 11;12(1):79. doi: 10.1186/s13073-020-00777-8. PubMed
Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. Daniloski Z, Jordan TX, Wessels HH, Hoagland DA, Kasela S, Legut M, Maniatis S, Mimitou EP, Lu L, Geller E, Danziger O, Rosenberg BR, Phatnani H, Smibert P, Lappalainen T, tenOever BR, Sanjana NE. Cell. 2020 Oct 24. pii: S0092-8674(20)31394-5. doi: 10.1016/j.cell.2020.10.030. PubMed

Associated Plasmids

Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells. Toropov AL, Deryabin PI, Shatrova AN, Borodkina AV. Int J Mol Sci. 2023 Sep 14;24(18):14089. doi: 10.3390/ijms241814089. PubMed
Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer. Spisak S, Chen D, Likasitwatanakul P, Doan P, Li Z, Bala P, Vizkeleti L, Tisza V, De Silva P, Giannakis M, Wolpin B, Qi J, Sethi NS. bioRxiv [Preprint]. 2024 Jan 17:2023.06.21.545895. doi: 10.1101/2023.06.21.545895. PubMed
Identifying regulators of aberrant stem cell and differentiation activity in colorectal cancer using a dual endogenous reporter system. Spisak S, Chen D, Likasitwatanakul P, Doan P, Li Z, Bala P, Vizkeleti L, Tisza V, De Silva P, Giannakis M, Wolpin B, Qi J, Sethi NS. Nat Commun. 2024 Mar 12;15(1):2230. doi: 10.1038/s41467-024-46285-w. PubMed

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