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Addgene

pHCKan-phoAp-GFPuv
(Plasmid #139072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMART-HC Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; DLF_R002
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phoAp-GFPuv
  • Species
    E. coli

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCKan-phoAp-GFPuv was a gift from Michael Lynch (Addgene plasmid # 139072 ; http://n2t.net/addgene:139072 ; RRID:Addgene_139072)

  • For your References section:

    Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli. Moreb EA, Ye Z, Efromson JP, Hennigan JN, Menacho-Melgar R, Lynch MD. ACS Synth Biol. 2020 Jun 19;9(6):1483-1486. doi: 10.1021/acssynbio.0c00182. Epub 2020 May 27. 10.1021/acssynbio.0c00182 PubMed 32353228
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