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Addgene

pLJC5-3XHA-EGFP-PEX26
(Plasmid #139054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJC5
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3XHA-EGFP-PEX26
  • Promoter UBC
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcorI (not destroyed)
  • 5′ sequencing primer TGAAGCTCCGGTTTTGAACT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJC5-3XHA-EGFP-PEX26 was a gift from David Sabatini (Addgene plasmid # 139054 ; http://n2t.net/addgene:139054 ; RRID:Addgene_139054)

  • For your References section:

    A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells. Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM. iScience. 2020 May 22;23(5):101109. doi: 10.1016/j.isci.2020.101109. Epub 2020 Apr 28. 10.1016/j.isci.2020.101109 PubMed 32417403
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