pXF90
(Plasmid
#139044)
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PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneZero blunt TOPO
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 3519
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehalotag::HDEL (includes STOP codon, PATC introns)
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXF90 was a gift from Erik Griffin (Addgene plasmid # 139044 ; http://n2t.net/addgene:139044 ; RRID:Addgene_139044) -
For your References section:
SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors. Fan X, Henau S, Feinstein J, Miller SI, Han B, Frokjaer-Jensen C, Griffin EE. G3 (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 10.1534/g3.119.400822. 10.1534/g3.119.400822 PubMed 31848219