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Addgene

pJF5
(Plasmid #139031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Zero blunt TOPO
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 3519
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gfp (no STOP codon, PATC introns)
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF5 was a gift from Erik Griffin (Addgene plasmid # 139031 ; http://n2t.net/addgene:139031 ; RRID:Addgene_139031)

  • For your References section:

    SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors. Fan X, Henau S, Feinstein J, Miller SI, Han B, Frokjaer-Jensen C, Griffin EE. G3 (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 10.1534/g3.119.400822. 10.1534/g3.119.400822 PubMed 31848219
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