pXF87
(Plasmid
#139027)
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Purpose(Empty Backbone) MosSCI backbone for MosSCI-SapTrap assembly
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneoriginal backbone pCFJ350; 2 unwanted SapI sites mutated, 2 SapI sites introduced for SapTrap assembly
- Backbone size (bp) 7534
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXF87 was a gift from Erik Griffin (Addgene plasmid # 139027 ; http://n2t.net/addgene:139027 ; RRID:Addgene_139027) -
For your References section:
SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors. Fan X, Henau S, Feinstein J, Miller SI, Han B, Frokjaer-Jensen C, Griffin EE. G3 (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 10.1534/g3.119.400822. 10.1534/g3.119.400822 PubMed 31848219
