HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector
(Plasmid
#139015)
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Purpose(Empty Backbone) To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139015 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
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Backbone manufacturer4100
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- HA-MAO (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CCCAAGCTTGATTTAG (SP6) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The vector contains a mitochondrial outer membrane targeting sequence from monoamine oxidase (MAO).
An HA-MAO fragment was amplified by PCR using a ratGM130ΔCterm (1-872L)-HA-MAO plasmid (pMX0050), kindly provided by Dr. Sean Munro at MRC.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector was a gift from Ken-Ichi Takemaru (Addgene plasmid # 139015 ; http://n2t.net/addgene:139015 ; RRID:Addgene_139015)
