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Addgene

pTub-alpha-1-mtGFP
(Plasmid #138981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    membrane targeted EGFP (mtGFP)
  • Mutation
    EGFP (added membrane targeting domain from GAP43).

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTub-alpha-1-mtGFP was a gift from Frank Bradke & Sebastián Dupraz (Addgene plasmid # 138981 ; http://n2t.net/addgene:138981 ; RRID:Addgene_138981)

  • For your References section:

    Axon Growth of CNS Neurons in Three Dimensions Is Amoeboid and Independent of Adhesions. Santos TE, Schaffran B, Broguiere N, Meyn L, Zenobi-Wong M, Bradke F. Cell Rep. 2020 Jul 21;32(3):107907. doi: 10.1016/j.celrep.2020.107907. 10.1016/j.celrep.2020.107907 PubMed 32698008
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