pQP-2376
(Plasmid
#138974)
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PurposeLight-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQP-2376 was a gift from Vladislav Verkhusha (Addgene plasmid # 138974 ; http://n2t.net/addgene:138974 ; RRID:Addgene_138974) -
For your References section:
Optogenetic regulation of endogenous proteins. Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV. Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4. 10.1038/s41467-020-14460-4 PubMed 32001718
Map uploaded by the depositor.
