pHD-DsRed-kkv-3::NG
(Plasmid
#138960)
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PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHD-DsRed
- Backbone size w/o insert (bp) 4048
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Vector typean editing vector for Drosophila
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3' end of kkv and neon green
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Alt nameChitin synthase
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SpeciesD. melanogaster (fly)
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GenBank IDNM_079509.3
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Entrez Genekkv (a.k.a. Dmel_CG2666, Blimp, CG2666, CS-1, CS-1/kkv, ChSB, DmCS-1, DmeCHS1, DmeChSB, Dmel\CG2666, Kkv, blimp, chitin synthase, l(3)82Fh)
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Tag
/ Fusion Protein
- Neon Green fused to C terminus of Kkv
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AAGGCTCAGAAAGAC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe vector was obtained from Addgene ((#63166).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/718841v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHD-DsRed-kkv-3::NG was a gift from Paul Adler (Addgene plasmid # 138960 ; http://n2t.net/addgene:138960 ; RRID:Addgene_138960) -
For your References section:
The localization of chitin synthase mediates the patterned deposition of chitin in developing Drosophila bristles. Adler PN. bioRxiv 2020 10.1101/718841