pUAS-kkv-mos::NG
(Plasmid #138958)

• Purpose: Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4
• Depositing Lab: Paul Adler
• Publication: Adler et al bioRxiv 2020

Backbone

• Vector backbone: pUAST-attb
• Backbone size w/o insert (bp): 8489
• Vector type: Insect Expression ; UAS transgene vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kkv
• Alt name: chitin synthase
• Species: D. melanogaster (fly)
• Insert Size (bp): 5553
• Mutation: 8 bp that are in the region that encodes the catalytic domain are mutated to encode the mosquito sequence.
• GenBank ID: NM_079509.3
• Entrez Gene: NEWENTRY
• Promoter: UAS
• Tag / Fusion Protein:
  • Neon Green fused to C terminus of Kkv

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: CTGGGTGCTGCCTGCGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I obtained the Neon Green DNA from Allele Biotech and the kkv cDNA from the fly genome project.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/718841v3 for bioRxiv preprint.