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Addgene

pUAS-kkv-OH
(Plasmid #138956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-attb
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kkv with C terminal epitope tag
  • Alt name
    Chitin synthase
  • Species
    D. melanogaster (fly)
  • GenBank ID
    NM_079509.3
  • Entrez Gene
    kkv (a.k.a. Dmel_CG2666, Blimp, CG2666, CS-1, CS-1/kkv, ChSB, DmCS-1, DmeCHS1, DmeChSB, Dmel\CG2666, Kkv, blimp, chitin synthase, l(3)82Fh)
  • Promoter UAS
  • Tag / Fusion Protein
    • ollas and his-6 added to C terminus of protein coding region (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer CTGGGTGCTGCCTGCGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The kkv cDNA came from the Drosophila genome project.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/718841v3 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-kkv-OH was a gift from Paul Adler (Addgene plasmid # 138956 ; http://n2t.net/addgene:138956 ; RRID:Addgene_138956)

  • For your References section:

    The localization of chitin synthase mediates the patterned deposition of chitin in developing Drosophila bristles. Adler PN. bioRxiv 2020 10.1101/718841
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