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Addgene

pRHA12
(Plasmid #138942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    riboregulated sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1125

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer VF2
  • 3′ sequencing primer VR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRHA12 was a gift from Guillermo Rodrigo (Addgene plasmid # 138942 ; http://n2t.net/addgene:138942 ; RRID:Addgene_138942)

  • For your References section:

    Binary addition in a living cell based on riboregulation. Rosado A, Cordero T, Rodrigo G. PLoS Genet. 2018 Jul 19;14(7):e1007548. doi: 10.1371/journal.pgen.1007548. eCollection 2018 Jul. 10.1371/journal.pgen.1007548 PubMed 30024870
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