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Addgene

pRHA40
(Plasmid #138941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    riboregulated mRFP1
  • Species
    Synthetic
  • Insert Size (bp)
    2189

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRHA40 was a gift from Guillermo Rodrigo (Addgene plasmid # 138941 ; http://n2t.net/addgene:138941 ; RRID:Addgene_138941)

  • For your References section:

    Binary addition in a living cell based on riboregulation. Rosado A, Cordero T, Rodrigo G. PLoS Genet. 2018 Jul 19;14(7):e1007548. doi: 10.1371/journal.pgen.1007548. eCollection 2018 Jul. 10.1371/journal.pgen.1007548 PubMed 30024870
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