RCAS(A) cScx EnR (CT#745)
(Plasmid
#13890)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScleraxis
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Alt nameScx
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Alt nameEnR
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SpeciesG. gallus (chicken)
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Entrez GeneSCX (a.k.a. SCXB)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Scleraxis fused to the Engrailed repressor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS(A) cScx EnR (CT#745) was a gift from Cliff Tabin (Addgene plasmid # 13890 ; http://n2t.net/addgene:13890 ; RRID:Addgene_13890) -
For your References section:
A somitic compartment of tendon progenitors. Brent AE, Schweitzer R, Tabin CJ. Cell. 2003 Apr 18. 113(2):235-48. 10.1016/S0092-8674(03)00268-X PubMed 12705871