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pPD027 Reporter Template v1 (Near Spaced)
(Plasmid #138751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD027 Reporter Template v1 (Near Spaced)
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compatible with BsaI Golden Gate system. To insert promoter, use annealed primers or PCR product in a Golden Gate Reaction with BsaI. This is an early version of the template and uses palindromic sticky ends, increasing the risk of multiple insertion. Generally, template v3 should be used to avoid this risk. However, this template can be used for comparing close/mid/far spaced promoters with pPD032 and pPD033. Insertion site is 33bp from minimal promoter. Leonard Lab plasmid reference number: L1268

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD027 Reporter Template v1 (Near Spaced) was a gift from Joshua Leonard (Addgene plasmid # 138751 ; http://n2t.net/addgene:138751 ; RRID:Addgene_138751)

  • For your References section:

    The COMET toolkit for composing customizable genetic programs in mammalian cells. Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard JN. Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5. 10.1038/s41467-019-14147-5 PubMed 32034124
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